Cell cycle analysis of asynchronous cell populations by flow cytometry using bromodeoxyuridine label and Hoechst-propidium iodide stain
β Scribed by Dr. Michael G. Ormerod; Manfred Kubbies
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 682 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Flow cytometry was used to measure cell cycle parameters in Solanum aviculare plant cell suspensions. Methods for bromodeoxyuridine (BrdU) labeling of plant nuclei were developed so that cell cycle times and the proportion of cells participating in growth could be determined as a function of culture
This study compares the cell cycle distribution in rat thymocytes obtained by means of bromodeoxyuridine (BrdUrd) labeling of S-phase cells and the analysis of the S-phase fraction obtained according t o the technique ofvindelov et al. (Cytometry 3332-338,1983). The proportion of BrdUrd-labeled cell
## Abstract ## Background: The analysis of cells from multiple experimental groups by multiparameter flow cytometry leads to the generation of complex data sets, for which adequate analysis tools are not commonly available. We report here that software designed for transcriptomics applications can
The simultaneous detection of the green fluorescent protein (GFP) and DNA content using propidium iodide (PI) by flow cytometry is made difficult because of the unique nature of these 2 fluorogenic reagents. For PI to enter cells efficiently and to stain DNA quantitatively, the cells must first be p