Use of t-butyldimethylsilylation in the gas chromatographic/mass spectrometric analysis of physiologic compounds found in plasma using electron-impact ionization

✍ Scribed by W. Frederick Schwenk; Peter J. Berg; Bernard Beaufrere; John M. Miles; Morey W. Haymond

• Book ID: 102987136
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 503 KB
• Volume: 141
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90431-7

✦ Synopsis

The use of N-methyl-N-(t-butyldimethylsilyl)trifluoroacetamide to prepare the t-butyldimethylsilyl derivatives of a number of organic compounds (selected amino acids, a-keto acids, ketone bodies, free fatty acids, urea, glycerol, lactate, and pyruvate) is reported. These derivatives are particularly useful for gas chromatographic/mass spectrometric analysis involving the use of stable isotopes and selected ion monitoring, since a peak of sufficient abundance at 57 mass/ charge units below the molecular ion was always present, and was the result of the loss of one t-butyl group. In each case, this fragment contained the entire skeleton of the original compound, which permitted easy analysis using electron-impact ionization of these compounds alone or when labeled with stable isotopes in any nonexchangeable position.