Gas chromatographic mass spectrometric determination of 1,2,3-propanetrioltrinitrate (nitroglycerin) in human plasma using the nitrogen-15 labelled compound as internal standard

✍ Scribed by A. Gerardin; D. Gaudry; D. Wantiez

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 300 KB
• Volume: 9
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200090805

✦ Synopsis

A method for the quantitative determination of 1,2,3-propanetrioltrinitrate (nitroglycerin) in human plasma by gas chromatography mass spectrometry has been developed. After addition of 1,2,3propanetriol(I5N)trinitrate as internal standard, plasma (1 ml) is extracted by a mixture of pentane and methyl acetate ( 90: 10). The extracts are purified by partition between, first, the extraction solvent and a mixture of acetonitrile and water (60:40) and, secondly, the resultant aqueous phase and benzene. The solvent is evaporated to a small volume before injection. The fragment ions at m / z 46 and 47 are monitored for the measurement of the [NOz]+ and ['sNOz]' ions using electron impact ionization. The mean recovery (%) I S D in blind plasma samples spiked with amounts in the concentration range 0.35-3.52 nmol I-', was 97.4 f 9.0 (n = 58). Within a day, recovery experiments gave rise to coefficients of variation of 9.6,6.4 and 2.7% at the levels 0.44,0.88 and 11 nmol I-', respectively. Concentrations in plasma down to about 0.2 nmol I-' (50 pg ml-I) could be estimated. Percent recovery of duplicate determinations in the range 0.5-1.96 nmol I-' f SD was 99.4 f 6.0 (n = 80).