When using weakly interacting ligands in affinity chromatography, it is possible to take advantage of a true chromatographic process in the separation, as compared with traditional affinity chromatography which is rather an on/off process. In this work, weak monoclonal antibodies were immobilized on
β¦ LIBER β¦
Use of steroid glycosides for the affinity chromatography of cholesterol
β Scribed by I. V. Berezin; I. P. Andrianova; Ya. I. Lapuk; L. B. Alekseeva; P. K. Kintya; N. E. Mashchenko
- Book ID
- 112376328
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 370 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0009-3130
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The use of commercially available 5'-UTP-agarose as an affinity chromatography resin for RNase has been described. It was shown that at pH 5.3, 0.025 M piperazine-HCI buffer was effective for the adsorption of active RNase A and exhibited little nonbiospecific binding as has been shown earlier for S