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Use of reiterative selection for defining protein—nucleic acid interactions

✍ Scribed by Michel M Ouellette; Woodring E Wright


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
879 KB
Volume
6
Category
Article
ISSN
0958-1669

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Fluorescamine is a useful reagent in monitoring protein-DNA interactions only if a convenient method of separating the complex from free protein is available. Sedimentation of the complex provides such a method at least in the case of histone-like proteins capable of extensive interaction with DNA.