Use of pure-absorption-phase 31P/1H 2D COLOC NMR spectra for assignment of 31P signals of oligonucleotides
โ Scribed by Josepha M Fu; Stephen A Schroeder; Claude R Jones; Robert Santini; David G Gorenstein
- Publisher
- Elsevier Science
- Year
- 1988
- Weight
- 383 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0022-2364
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Two-dimensional 1H-31P correlation spectroscopy and relaxation time measurements of intact diseased pancreas were performed in order to identify an unknown peak appearing at -0.18 +/- 0.04 ppm in a 31P NMR spectrum of taurocholate-induced experimental acute pancreatitis in rats. The signal was ident
Assignment of all 31P resonances of a 16 base-pair DNA duplex, Sd(CTGCTCACTTTCCAGG)Y Sd(CCTGGAAAGTGAGCAG)Y, related to the DNA KB site of the HIV-1 LTR, was determined by 2D heteronuclear inverse NMR spectroscopy (HSQC-TOCSY and heteronuclear COSY). 'J(H3'-P) coupling constants for most of the oligo