Use of polybuffer as carrier ampholytes in mixed-bed immobiline gels for isoelectric focusing
β Scribed by Thierry Rabilloud; Barbara Barzaghi; Pier Giorgi Righetti
- Book ID
- 118387867
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 670 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0165-022X
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract A simple technique for polyacrylamide gel isoelectric focusing of Ξ±~1~βantitrypsin (PI) using thin, highly crossβlinked gels, restricted pHβrange carrier ampholytes and a separator was developed. This method enables easy typing of all known PI phenotypes including the four PI\*M subtype
Isoelectric points at 25" and 4" of some carrier ampholytes containing different protoQtic groups and dissociation constants at 25" of some carboxylic acids and alkyl-substituted ammonium ions have been determined in water and in sucrosewater, glycerol-water and ethylene glycol-water mixtures. Thes