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Reproducibility of polypeptide spot positions in two-dimensional gels run using carrier ampholytes in the isoelectric focusing dimension

✍ Scribed by Dr. Mary F. Lopez; Wayne F. Patton


Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
612 KB
Volume
18
Category
Article
ISSN
0173-0835

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The proteome of the yeast Saccharomyces cerevisiae was analysed by two-dimensional (2D) polyacrylamide gel electrophoresis utilizing a non-linear immobilized pH gradient (3-10) in the first-dimensional separation. Cells were labelled by [ 35 S]methionine incorporation in the respiro-fermentative pha