Use of phosphorothioate analogs of poly(dA-dT) · poly(dAdT) to study steroidal-diamine induced conformational change in poly(dA-dT) · poly(dA-dT)
✍ Scribed by J.William Suggs; Donna A. Taylor
- Book ID
- 115917130
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 432 KB
- Volume
- 189
- Category
- Article
- ISSN
- 0014-5793
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Raman spectra have been recorded for poly (dG-dT) . poly(dAdC) and poly (dA-dT) . poly(dA-dT) in low salt and at high concentrations of CsF. Poly(dGdT)poly (dAdC) shows no change in the 682-cm-I guanine mode, demonstrating the absence of the Zstructure at high salt. The 790-cm-I phosphodiester symm
Poly(dAdT).poly(dAdT) undergoes a reversible conformational transition in the presence of Co(NH&+ or spermidine in low salt (10 mM NaCl + 1 mM Na cacodylate). This transition is similar, as judged by changes in the CD spectrum, to the B-to-X transition of the polymer provoked by alcohol and Cs+ For