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Raman spectroscopic elucidation of DNA backbone conformations for poly(dG-dT) · poly(dA-dC) and poly(dA-dT) · poly(dA-dT) in CsF solution

✍ Scribed by Stephen P. A. Fodor; Philip A. Starr; Thomas G. Spiro

Publisher
Wiley (John Wiley & Sons)
Year
1985
Tongue
English
Weight
417 KB
Volume
24
Category
Article
ISSN
0006-3525

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✦ Synopsis

Raman spectra have been recorded for poly (dG-dT) . poly(dAdC) and poly (dA-dT) .

poly(dA-dT) in low salt and at high concentrations of CsF. Poly(dGdT)poly (dAdC) shows no change in the 682-cm-I guanine mode, demonstrating the absence of the Zstructure at high salt. The 790-cm-I phosphodiester symmetric stretch, however, shifts up 5 cm-I in 4.3M CsF, suggesting a slight conformational change, associated with ion binding or hydration changes. Poly(dA-dT) poly(dAdT) shows an additional broad band at 816 cm-', attributed to the phosphodiester modes associated with the C3'-endo deoxyribose units in the alternating B-structure. In this case, both the 841-and the 816-cm-I asymmetric phosphodiester stretches, associated with the C2'-and C3'-endo units, shift down on addition of CsF in a sequential manner. Correlation of this sequence with that previously observed for the two 31P-nmr resonances, establishes that the phosphodiester stretching frequencies depend on the conformation of the 5'-sugar, and not on the 3'sugar.

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