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Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans

โœ Scribed by Oldrich Janiczek; Blanka Pokorna; Jana Zemanova; Martin Mandl


Book ID
118471270
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
160 KB
Volume
117
Category
Article
ISSN
0168-1656

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โœ Y. Kroviarski; S. Cochet; C. Vadon; A. Truskolaski; P. Boivin; O. Bertrand ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 429 KB

The screening procedure described in the preceding paper allowed a practical purification procedure to be devised that was automated for human 6-phosphogluconate dehydrogenase. The purification needed only two chromatographic steps, first on immobilized Procion Blue HE-GN and then on Phenyl-Sepharos