Use of high-performance liquid chromatography in the separation of flavonol glycosides and flavonol sulphates

✍ Scribed by J.B. Harborne; M. Boardley

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 627 KB
• Volume: 299
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97853-7

✦ Synopsis

Forty flavonol glycosides have been separated by reversed-phase high-performance liquid chromatography (HPLC) on a Ca column by gradient elution with methanol-acetic acid-water mixtures. The results indicate that relative retention times are a useful adjunct for purposes of characterisation. Retention times are inversely correlated with increasing glycosylation, although the position of glycosylation in the flavonol moiety has a significant effect on mobility. The value of HPLC as an additional criterion for characterisation is illustrated by the identification of the quercetin arabinoside of Fueniculum vulgare as guaijaverin. Flavonol sulphates tend to overlap with glycosides under the above conditions but they can be separated by ion-pairing with tetrabutylammonium phosphate. HPLC of a crude extract of Oenanthe crocaru leaves provided quantitative measurements of the relative amounts of sulphated and unsulphated flavonoids.