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Influence of organic modifiers on the isocratic reversed-phase high-performance liquid chromatography of flavonol glycosides from brewer's hops

✍ Scribed by I. McMurrough; G.P. Hennigan

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
520 KB
Volume
258
Category
Article
ISSN
1873-3778

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✦ Synopsis

The elution order in reversed-phase high-performance liquid chromatography of certain flavonol glycosides found in hops was influenced by the concentration of tetrahydrofuran (THF) used under isocratic conditions. Whereas acceptable resolution of all compounds of interest was obtained with the appropriate water-THF mixtures, solvent mixtures containing methanol instead of THF were much less satisfactory. The solvent strength of acetic acid, when included in eluent mixtures was found to be greater than that of THF, which was in turn greater than that of methanol.

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