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Use of gene fusions to isolate promoter mutants in the transfer RNA gene tyrT of Escherichia coli

โœ Scribed by Michael L. Berman; Jon Beckwith


Book ID
118918162
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
950 KB
Volume
130
Category
Article
ISSN
0022-2836

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Fusion of the lac genes to the promoter
โœ Murgier, Maryse ;Gharbi, Skander ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Springer ๐ŸŒ English โš– 418 KB

Strains of Escherichia coli K12 were isolated in which the lac structural genes were fused to the promoter for the aminopeptidasee N structural gene (pepN). Although this enzyme is constitutively produced, the differential rate of synthesis is increased about 4-fold upon phosphate starvation. The pe