✦ LIBER ✦

Fusion of the lac genes to the promoter for the aminopeptidase N gene of Escherichia coli

✍ Scribed by Murgier, Maryse ;Gharbi, Skander

Publisher: Springer
Year: 1982
Tongue: English
Weight: 418 KB
Volume: 187
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00331136

No coin nor oath required. For personal study only.

✦ Synopsis

Strains of Escherichia coli K12 were isolated in which the lac structural genes were fused to the promoter for the aminopeptidasee N structural gene (pepN). Although this enzyme is constitutively produced, the differential rate of synthesis is increased about 4-fold upon phosphate starvation. The pepN-lac fusions were shown to respond to phosphate specific regulatory signals. A plaque forming lambda transducing phage bearing the pepN-lac fusion was isolated. This phage was used to prove genetically the fusion of lac genes to the promoter for the aminopeptidase. These results demonstrate a control at the transcriptional level of aminopeptidase synthesis.

📜 SIMILAR VOLUMES

Fusion of the lac genes to the promotor

Fusion of the lac genes to the promotor for the cytidine deaminase gene of Escherichia coli K-12

✍ Josephsen, Jytte ;Hammer-Jespersen, Karin 📂 Article 📅 1981 🏛 Springer 🌐 English ⚖ 517 KB

Phage Mu has been inserted into the structural gene for cytidine deaminase (cdd). By the use of phage 2 (lac, Mu) the promoter for the cdd gene has been fused to lacZ. In these strains lacZ expression is regulated by the cytR repressor protein and is therefore induced by cytidine. The fusion strains

Physical mapping of the gene for aminope

Physical mapping of the gene for aminopeptidase N in Escherichia coli K12

✍ Bally, M. ;Murgier, M. ;Tommassen, J. ;Lazdunski, A. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 139 KB

The pepN gene has been cloned into the multicopy plasmid pBR322. The restriction map of the insert was established and the gene was localized. By comparison with the restriction map of the plasmid pJP30 bearing the ompF region, it has been possible to order the ompF, asnS, and pepN genes. The ompF a

Cloning and orientation of the gene enco

Cloning and orientation of the gene encoding aminopeptidase N in Escherichia coli

✍ Bally, Marc ;Murgier, Maryse ;Lazdunski, Andrée 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 450 KB

The pepN gene, that encodes aminopeptidase N in Escherichia coli, has been cloned into the multicopy plasmid pBR322. Expression of the cloned pepN gene results in overproduction of the enzyme. The restriction map of the 6.7 Kb insert was established and the gene was further localized by analysis of

Gene fusions to the ptsM/pel locus of Es

Gene fusions to the ptsM/pel locus of Escherichia coli

✍ Palva, E. Tapio ;Saris, Per ;Silhavy, Thomas J. 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 731 KB

We have constructed gene fusions between ptsM/pel and lacZ. These fusions affect both phenotypes assigned to the ptsM/pel locus (at 40 min), namely, no growth on mannose or glucosamine and inhibition of the penetration of bacteriophage lambda DNA, as well as that of other lambdoid phages such as Hy-

Characterisation of the promoter for the

Characterisation of the promoter for the LexA regulated sulA gene of Escherichia coli

✍ Cole, Stewart T. 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 529 KB

The sulA gene of Escherichia coli, which encodes an inducible inhibitor of cell division, plays a role in the SOS response. Its expression, like that of other SOS genes, is repressed by the LexA protein. This paper reports the identification and characterisation of the promoter for the cloned sulA g

The inhibition of Escherichia coli lac o

The inhibition of Escherichia coli lac operon gene expression by antigene oligonucleotides–mathematical modeling

✍ Biao Cheng; Ronald L. Fournier; Patricia A. Relue 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 124 KB 👁 2 views