The optimization and validation of a cleaning procedure The use of dynamic contact angle (DCA) analysis for the evaluto remove biological material from contaminated surfaces ation of cleaning procedures is described. Stainless-steel, glass, are by no means trivial tasks. Yet even before such optimiz
โฆ LIBER โฆ
Use of endoproteinase Lys-C from Lysobacter enzymogenes in protein sequence analysis
โ Scribed by Peter A. Jekel; Wicher J. Weijer; Jaap J. Beintema
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 477 KB
- Volume
- 134
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
Endoproteinase Lys-C from Lysobacter enzymogenes, which is commercially available, proved to be useful in the determination of primary structures of proteins. The enzyme preferentially cleaves at the carboxyl side of lysine residues.
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Non-porous reversed phase (NPRP) high-performance liquid chromatography (HPLC) has been used as a rapid method to separate proteins from whole cell lysates of human erythroleukemia (HEL) cells. Using phosphate-buffered saline (PBS) as a lysis buffer to extract proteins from HEL cells, more than 100