Use of Dynamic Contact Angle Profile Analysis in Studying the Kinetics of Protein Removal from Steel, Glass, Polytetrafluoroethylene, Polypropylene, Ethylenepropylene Rubber, and Silicone Surfaces
✍ Scribed by John Davies; Caroline S. Nunnerley; Anneli C. Brisley; John C. Edwards; Stewart D. Finlayson
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 140 KB
- Volume
- 182
- Category
- Article
- ISSN
- 0021-9797
No coin nor oath required. For personal study only.
✦ Synopsis
The optimization and validation of a cleaning procedure The use of dynamic contact angle (DCA) analysis for the evaluto remove biological material from contaminated surfaces ation of cleaning procedures is described. Stainless-steel, glass, are by no means trivial tasks. Yet even before such optimizapolytetrafluoroethylene, polypropylene, ethylenepropylene rubber, tion studies can take place it is necessary to define the term and silicone substrates were soaked in albumin solution to contamclean. While the level of cleanliness required is dependent inate their surfaces. The kinetics of removal of the adsorbed proon the subsequent use of the manufactured product, the level tein by immersion in a cleaning solution (NaOH/Triton X-100) of cleanliness required will determine which analytical was evaluated by DCA adsorption profile analysis to determine method is suitable for the task in hand. optimum soak times in the cleaning solution. Soak times are shown Traditional surface analytical techniques such as X-ray to vary significantly with the type of material. While glass and photoelectron spectroscopy (XPS) and time-of-flight secsilicone substrates are cleaned within a few minutes, the soak time for stainless steel under the experimental conditions employed is ondary ion mass spectrometry (ToF-SIMS) appear to be shown to be on the order of an hour.