Use of DOP-PCR for amplification and labeling of BAC DNA for FISH
β Scribed by Darouich, S; Popovici, C; Missirian, C; Moncla, A
- Book ID
- 115548468
- Publisher
- Informa plc
- Year
- 2011
- Tongue
- English
- Weight
- 154 KB
- Volume
- 87
- Category
- Article
- ISSN
- 1052-0295
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## Abstract We have designed DOPβPCR primers specifically for the amplification of large insert clones for use in the construction of DNA microarrays. A bioinformatic approach was used to construct primers that were efficient in the general amplification of human DNA but were poor at amplifying __E
The standard comparative genomic hybridization (CGH) protocol relies on availability of macroscopic tumor samples, which do not contain too much interfering normal cells. Recently, CGH after universal amplification of genomic DNA with degenerate oligonucleotide primed PCR (DOP-PCR) has been used to