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DOP-PCR Amplification of Probe DNA for Whole-Mount FISH in Drosophila

โœ Scribed by Dernburg, A. F.


Book ID
121375638
Publisher
Cold Spring Harbor Laboratory Press
Year
2012
Tongue
English
Weight
267 KB
Volume
2012
Category
Article
ISSN
1940-3402

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The standard comparative genomic hybridization (CGH) protocol relies on availability of macroscopic tumor samples, which do not contain too much interfering normal cells. Recently, CGH after universal amplification of genomic DNA with degenerate oligonucleotide primed PCR (DOP-PCR) has been used to