𝔖 Bobbio Scriptorium
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Use of dental wax for the study of insect behavior by scanning electron microscopy

✍ Scribed by Kenneth M. Bart; Ernest H. Williams

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
335 KB
Volume
26
Category
Article
ISSN
1059-910X

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✦ Synopsis

While using scanning electron microscopy (SEM) to investigate the impact of ovipositing butterflies on the leaf surface, it became clear to us that the complex topography of natural leaf surfaces, including the crystalline structure of surface waxes (as shown in Hallam & Juniper, 1971), makes it difficult to find and recognize marks made by insects. In consequence, we devised a new technique, using an artificial leaf surface, for viewing such marks.

Dental wax (Hygenic Extra Tough Base Plate Wax, Pelco Inc. #109) was cut into 1 sq. cm pieces, and one

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