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Use of Cyclodextrin-Cholesterol Complex as a Primary Standard in Cholesterol Analysis

✍ Scribed by N. Karuppiah; P.B. Kaufman; S.A. Kapustka; A. Sharma

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
234 KB
Volume
47
Category
Article
ISSN
0026-265X

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✦ Synopsis

Cholesterol is an analyte commonly measured in various biological fluids. Enzymic procedures have virtually replaced the chemical methods for cholesterol determinations. Standardization of cholesterol assays often involve the use of solutions of this steroid containing organic solvents and/or detergents, or alternatively, the use of secondary standards. The present report shows the preparation of a primary cholesterol standard by its complexation with a chemically modified cyclodextrin, hydroxylpropyl (\beta)-cyclodextrin. The use of cyclodextrin for cholesterol solubilization is not deleterious to the enzymes and other constituents present in reagents for cholesterol analysis. Such a standard is biocompatible, stable, and safe and offers an excellent alternative to the utilization of organic solvents or detergents. & 1993 Academic Press. Inc.

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