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Use of cRNA digoxigenin-labelled probes for detection of enteroviruses in humans and in the environment

✍ Scribed by Florence Fuchs; Isabelle Leparc; Helena Kopeck; Daniel Garin; Michéle Aymard

Book ID
119115272
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
961 KB
Volume
42
Category
Article
ISSN
0166-0934

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Subgenomic fragments of cDNA from poliovirus type 1 were inserted downstream from the SP6 or the T7 promoter in a Gemini riboprobe vector and their in vitro synthesized RNA transcripts were used as radiolabeled probes for the detection of enteroviral RNAs by molecular hybridization. The cRNA transcr