Detection of Hepatitis C Virus (HCV) RNA in Liver Tissue Specimens by in Situ Hybridization With Digoxigenin-Labeled cRNA Probes
β Scribed by Mittal, Sahil ;Abdulla, Nihal E. ;Eltorky, Mahmoud A. ;Ferguson, Monique R. ;Oommen, Smitha E. ;Li, Kui ;Wang, Nan ;Li, Guangyu
- Book ID
- 122228241
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 223 KB
- Volume
- 140
- Category
- Article
- ISSN
- 0016-5085
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The cellular localization of hepatitis C virus (HCV) RNA in liver tissue was studied by nonisotopic in situ hybridization using a digoxigenin-labeled cDNA probe created during a polymerase chain reaction on samples from 16 patients with chronic HCV infection. Hybridization signalswere recognized in
## Abstract A rapid technique for the detection of hepatitis C virus (HCV) RNA in liver section using nonisoβtopic in situ hybridization was described. Only 3 of the 10 patients seropositive for antibody to HCV and HCV RNA had detectable HCV RNA in the hepatocytes (1%, 2%, and 15% of cells positive