Use of a novel colour reaction in the chromatography of 6-oxo-prostaglandin F1a and related prostaglandins

✍ Scribed by F.B. Ubatuba

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 950 KB
• Volume: 161
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85224-9

✦ Synopsis

6-Oxo-prostaglandin FLa (6-0x0-PGF,,), derivatized by methylation, methoxylation and silylation, emerged from OV-1 and OV-17 silicone gas-liquid chromatographic columns as one major peak (equivalent chain length = 25.1). A standardization curve, constructed using pure 6-oxo-PGF,, enabled quantificatiorn down to 5 ng. Silicic acid column chromatography effectively separated 6-oxo-PGF,, (free acid) from all other major prostaglandins. On thin-layer chromatographic silica gel plates, 6-oxo-PGF,, was resolved as discrete spots, well separated from other prostaglandins when developed in 5 out of 7 solvent mixtures. The difference in rate of resolution between 6-oxo-PGF,, and the PGFs in the various solvents afforded another criterion for identification. 6-0x0-PGF,, produced a distinctive yellow chromogen on thin-layer chromatographic silica gel plates sprayed with an anisaldehyde-sulphuric acid reagent; the colour reaction is specific to 6-oxo-PGF1, and some structurally related prostaglandins.