Use of a mixed derivative for the gas chromatography and mass spectrometry of cytidine and its analogs

✍ Scribed by U.I. Krahmer; J.G. Liehr; K.J. Lyman; E.A. Orr; R.N. Stillwell; James A. McCloskey

• Book ID: 102982641
• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 508 KB
• Volume: 82
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90151-8

✦ Synopsis

Trimethylsilylation of cytidine and its analogs normally produces on gas chromatography a peak which is unusually broad and low and whose area is strongly affected by sample size. Addition of trace amounts (<I%) of N-trifluoroacetylimidazole to the silylation reaction mixture followed by standing 5 to 10 min produces the W-trifluoracetyl,O-persilyl derivative which is substantially more volatile and forms a sharp, symmetrical peak on gas chromatography. Since the mixed derivative is not formed from other common nucleosides the reaction provides a selective form of n&leoside or nucleotide modification that is potentially useful for characterization of cytosine-containing analogs by mass spectrometry.