Use of a Hepta-tyr glycopeptide antibiotic as chiral selector in capillary electrophoresis

✍ Scribed by Dr. Salvatore Fanali; Zeineb Aturki; Claudia Desiderio; Alessandra Boss; Pier Giorgio Righetti

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 867 KB
• Volume: 19
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150191036

✦ Synopsis

Use of a Hepta-tyr glycopeptide antibiotic as c h i d selector in capillary electrophoresis

A new glycopeptide antibiotic, MDL 63,, of the teicoplanin family, has been evaluated in capillary electrophoresis for the resolution of chiral compounds of pharmaceutical and environmental interest. Electrophoretic separations were carried out in a polyacrylamide-coated capillary using the partial filling-counter current mode with aqueous-organic buffers in the pH range 4-6. Experimental parameters affecting resolution, such as antibiotic concentration, buffer pH, organic modifier type and capillary temperature, were studied. The Hepta-tyr antibiotic exhibited a high enantiorecognition capability towards the studied compounds at very low concentrations (1-2 mg/mL). The optimum experimental conditions were achieved by using a buffer at pH 5 containing acetonitrile at 25°C.