The use of the o-phthalaldehyde reaction
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Stanley A. Robrish; Christopher Kemp; William H. Bowen
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Article
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1978
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Elsevier Science
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English
โ 618 KB
Alkaline hydrolysis of protein followed by reaction with o-phthalaldehyde has permitted the determination of less than 10 ng of protein fluorometrically. When intact proteins were analyzed with o-phthalaldehyde the reaction was less sensitive. This reaction has been applied to analysis of the protei