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Use of 15N labeling for automated three-dimensional sorting of cross peaks in protein 2D NMR spectra

✍ Scribed by Paul L Weber; Luciano Mueller


Book ID
107798255
Publisher
Elsevier Science
Year
1989
Weight
398 KB
Volume
81
Category
Article
ISSN
0022-2364

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Three-Dimensional Solid-State NMR Spectr
✍ Francesca M Marassi; Che Ma; Jennifer J Gesell; Stanley J Opella πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 188 KB

Uniformly 15 N-labeled samples of membrane proteins with helices aligned parallel to the membrane surface give two-dimensional PISEMA spectra that are highly overlapped due to limited dispersions of 1 H-15 N dipolar coupling and 15 N chemical shift frequencies. However, resolution is greatly improve