Uronic acid content in polysaccharides: Estimation by decarboxylation involving use of gas chromatography

✍ Scribed by L.P. Kosheleva; G.Y. Ilchenko; L.I. Glebko

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 240 KB
• Volume: 73
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90147-0

✦ Synopsis

A quantitative method for determining uranic acid content in different polysaccharides has been described. The decarboxylation reaction was run with 57% hydriodic acid (HI) in a small flask attached directly to the gas circuit of a chromatograph. After the reaction was completed, the resultant carbon dioxide was supplied to the chromatographic column. A sample uranic acid (0.5-10.0 pmol) yielded quantitative results with a relative standard deviation of 2%. The contribution of nonuronic materials to the error did not exceed 0.6%. The total analysis time amounted to 50-60 min.