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Uronic acid analysis by automated anion exchange chromatography

✍ Scribed by Mary Jane Spiro

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
266 KB
Volume
82
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid and sensitive assay for individual uranic acids has been developed based on their separation on a 0.5 X 22-cm column of Aminex A-25 in 0.12 M Tris-acetate buffer, pH 7.4. Quantification of these sugars is accomplished by coupling the column to the analytical portion of a Technicon sugar analyzer. Each determination is complete in 3 hr. and as little as 25 nmol of uranic acid can be measured with accuracy.

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