Hydrolysis of NAD by a nucleotide pyrophosphatase of renal membrane fractions has been reported previously. The aim of the present study was to localize this enzyme in the rat kidney. Nucleotide pyrophosphatase was assayed in glomeruli, in three parts of the proximal tubule and in four parts of the
Uromucoid in the rat: Its isolation, localization in the kidney and concentration in the urine
โ Scribed by Kirchner, C. ;Bichler, K.-H.
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 389 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0300-5623
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โฆ Synopsis
Based on the finding that rat uromucoid cross reacts with antihuman uromucoid, rat uromucoid was isolated by means of immune absorption chromatography. Using a monospecific antiserum obtained with the use of this preparation, uromucoid was shown to be present in the distal tublues of the kidney. A quantitative immunological determination was made from the urine of 30 normal rats.
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