Urinary 6βP-hydroxycortisol in humans: Analysis, biological variations, and reference ranges

✍ Scribed by Chul Lee

• Book ID: 103040578
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 527 KB
• Volume: 28
• Category: Article
• ISSN: 0009-9120
• DOI: 10.1016/0009-9120(94)00060-9

✦ Synopsis

Objective: To evaluate methods for analysis, biological variation, and reference ranges for 6~-hydroxycortisol in urine.

Methoda:

The urinary level of 613-hydroxycortisol (6~-OHF) was measured by reversed-phase high performance liquid chromatography.

Results:

The method was accurate and precise, as indicated by a recovery rate of 92-95% and less than 5.5% and 6.6% of coefficient of variation for within-run and between-run assay, respectively. The reference range of urinary 613-OHF was 187-1245 nmol/day in adults (20-60 years) and 103-915 nmol/day in the elderly (>61 years). No sigr, ificant day-to-day intrabiological variation was observed in a 5-day period. Diurnal rhythms of urinary 61~-OHF and urinary free cortisol (UFC) were quite parallel to each other, so that the ratio of 613-OHF to UFC remained constant through the day. Conclusion: An early morning urine specimen can provide the urinary ratio of 613-OHF to UFC as adequately as that determined from 24-h specimen.