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Uridine phosphorylase from Escherichia coli B.: Kinetic studies on the mechanism of catalysis

โœ Scribed by Alberto Vita; Charles Y. Huang; Guilio Magni

Book ID
115705730
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
364 KB
Volume
226
Category
Article
ISSN
0003-9861

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A one-step procedure for the purificatio
A one-step procedure for the purification of uridine phosphorylase from Escherichia coli
โœ Alberto Vita; Giulio Magni ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 300 KB

Adsorption to Matrex Gel Green A and successive elution with uridine provides a one-step procedure for the purification of uridine phosphorylase from Escherichia coli B. The homogeneous preparation of the enzyme can be obtained within a single day with an activity recovery above 80%.