Urea Facilitates Quantitative Estimation of DNA Damage in Situ by Preventing Its Degradation

✍ Scribed by F.E. Ahmed

• Book ID: 102561315
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 457 KB
• Volume: 210
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1192

✦ Synopsis

A buffer containing (7 \mathrm{~m}) urea was used to successfully prevent DNA degradation of in situ damaged fish skin and resulted in reproducible high molecular weight DNA. This treatment in combination with a quantitative gel electrophoresis technique permitted estimation of small changes induced in unlabeled DNA per unit molecular weight after treatment with a DNA damaging agent (e.g., ultraviolet irradiation). 1993 Academic Press, Inc.