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Estimation of the detection rate in STR analysis by determining the DNA degradation ratio using quantitative PCR

โœ Scribed by Kitayama, Tetsushi; Fujii, Koji; Nakahara, Hiroaki; Mizuno, Natsuko; Kasai, Kentaro; Yonezawa, Naoto; Sekiguchi, Kazumasa


Book ID
119318129
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
594 KB
Volume
15
Category
Article
ISSN
1344-6223

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A method combining gas chromatography and mass spectrometry (GC-MS) with multiple specific ion monitoring has been developed for the detection of S-methylcytosine and the quantitation of the ratio of methylcytosine to cytosine in DNA. The trimethylsilyl derivatives of cytosine and S-methylcytosine o