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Urea denaturation of barnase: pH dependence and characterization of the unfolded state

✍ Scribed by Pace, C. Nick; Laurents, Douglas V.; Erickson, Rick E.


Book ID
120667314
Publisher
American Chemical Society
Year
1992
Tongue
English
Weight
858 KB
Volume
31
Category
Article
ISSN
0006-2960

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Equilibrium unfolding of RNase Rs from R
✍ Rajashree A. Deshpande; M.Islam Khan; Vepatu Shankar πŸ“‚ Article πŸ“… 2003 πŸ› Elsevier Science 🌐 English βš– 778 KB

The conformational stability of RNase Rs was determined with chemical and thermal denaturants over the pH range of 1-10. Equilibrium unfolding with urea showed that values of D(1/2) (5.7 M) and DeltaG(H(2)O) (12.8 kcal/mol) were highest at pH 5.0, its pI and the maximum conformational stability of R