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Equilibrium unfolding ofA. nigerRNase: pH dependence of chemical and thermal denaturation

โœ Scribed by Gundampati Ravi Kumar; Anurag Sharma; Moni Kumari; Medicherla V. Jagannadham; Mira Debnath


Book ID
105946815
Publisher
Springer
Year
2011
Tongue
English
Weight
546 KB
Volume
40
Category
Article
ISSN
1432-1017

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Equilibrium unfolding of RNase Rs from R
โœ Rajashree A. Deshpande; M.Islam Khan; Vepatu Shankar ๐Ÿ“‚ Article ๐Ÿ“… 2003 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 778 KB

The conformational stability of RNase Rs was determined with chemical and thermal denaturants over the pH range of 1-10. Equilibrium unfolding with urea showed that values of D(1/2) (5.7 M) and DeltaG(H(2)O) (12.8 kcal/mol) were highest at pH 5.0, its pI and the maximum conformational stability of R