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Uptake of Escherichia coli DNA into HeLa cells enhanced by amphotericin B

โœ Scribed by VIJAYA KUMAR, B.; MEDOFF, GERALD; KOBAYASHI, GEORGE; SCHLESSINGER, DAVID

Book ID
109692159
Publisher
Nature Publishing Group
Year
1974
Tongue
English
Weight
278 KB
Volume
250
Category
Article
ISSN
0028-0836

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Enhanced uptake of donor DNA by Ca2+ tre
Enhanced uptake of donor DNA by Ca2+ treated Escherichia coli cells
โœ Sabelnikov, A.G. ;Avdeeva, A.V. ;Ilyashenko, B.N. ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Springer ๐ŸŒ English โš– 444 KB

The first steps in E. coli transformation have been studied. It was found that the cells attain the ability of enhanced uptake of donor exogenous DNA upon Ca2+ treatment. At saturating DNA concentrations the cells were capable to take up irreversibly about 6-10(8) dalton of donor DNA per cell (if to