Uptake and aminoacylation of exogenous transfer RNA by mouse leukemia cells
โ Scribed by Robert E. Gallagher; Christine A. Walter; Robert C. Gallo
- Book ID
- 118853223
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 507 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0006-291X
No coin nor oath required. For personal study only.
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Normal mouse spleen cells take up in vitro radioactively labeled immune RNA. RNA taken up is present in nuclei, polysomes, membranes and cytoplasm. About 20-40% of immune RNA is nonspecifically associated with cell surface. 45% of RNA taken up is degraded and reutilized inside the cells within 2 hou
Methionine-auxotrophic L1210 cells were used to study the effect of methotrexate (MTX) on methionine uptake and metabolism. MTX was shown to inhibit amino acid transport systems and cause a decrease of methionine uptake into L1210 cells. Conversely, a nonmetabolizable amino acid analogue reduced MTX