Updated Gene Map of Tobacco Chloroplast DNA
โ Scribed by T. Wakasugi; M. Sugita; T. Tsudzuki; M. Sugiura
- Book ID
- 110251624
- Publisher
- Springer
- Year
- 1998
- Tongue
- English
- Weight
- 58 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0735-9640
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We have determined the position of Spirodela oligorhiza chloroplast 4S RNA genes on the restriction fragment map of cp DNA, using purified in vitro[(32)P]-labeled 4S RNA. The overall organization of these genes is very similar to the organization of tRNAs on spinach cp DNA (Driesel et al. 1979).
The cytoplasmic and chloroplast tRNAs of Euglena gracilis Z strain were fractionated by twodimensional gel electrophoresis and identified by aminoacylation. Purified chloroplast tRNAs, labeled in vitro with 13zPl, were hybridized to endonuclease restriction fragments of chloroplast DNA, allowing the