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Fractionation and identification ofEuglena graciliscytoplasmic and chloroplastic tRNAs and mapping of tRNA genes on chloroplast DNA

โœ Scribed by M. Kuntz; M. Keller; E. J. Crouse; G. Burkard; J. H. Weil

Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
743 KB
Volume
6
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

The cytoplasmic and chloroplast tRNAs of Euglena gracilis Z strain were fractionated by twodimensional gel electrophoresis and identified by aminoacylation. Purified chloroplast tRNAs, labeled in vitro with 13zPl, were hybridized to endonuclease restriction fragments of chloroplast DNA, allowing the corresponding tRNA genes to be localized on the physical map of Euglena chloroplast DNA.

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