Unusual sterolic mixture, and 24-isopropylcholesterol, from the sponge Ciocalypta sp. reduce cholesterol uptake and basolateral secretion in Caco-2 cells

✍ Scribed by Hong Quang Do; Laurianne Van Landeghem; Gaëtane Wielgosz-Collin; Martin Takoudju; Jean-Michel Huvelin; Jean-Michel Kornprobst; Jean-Marie Bard; Gilles Barnathan; Hassan Nazih

• Book ID: 102305326
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 224 KB
• Volume: 106
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.22047

✦ Synopsis

Abstract

An unusual sterolic mixture (82.3% of 24‐isopropylated sterols) and its major component, 24‐isopropylcholesterol, isolated from a marine sponge, Ciocalypta sp. (Halichondriidae), reduce cholesterol uptake, basolateral secretion and ACAT‐2 mRNA expression and increase the expression of ABCA1 mRNA in Caco‐2 cells. The decreases of cholesterol uptake and secretion induced by 24‐isopropylcholesterol alone were more than that of both the sterolic mixture and β‐sitosterol. These data add a new sterol, 24‐isopropylcholesterol, to sterols that may reduce intestinal cholesterol absorption. J. Cell. Biochem. 106: 659–665, 2009. © 2009 Wiley‐Liss, Inc.