✍ Scribed by H. Dahlheim; P. Weber; I. Herold; K. Thurau
No coin nor oath required. For personal study only.
The present studies were undertaken to obtain a high concentration of renin-substrate.
Hog serum was fractionated by salting-out with graded increments of NaC1 concentrations at pt{ 3.1. A substrate concentration of 30 times that of the one in the original serum--in terms of ng angiotensin produced per minute and per mg protein--was obtained in a fraction resulting from an addition of 180--210 g NaC1 to 1 litre of serum.
A further 30 times purification was achieved through the treatment of the above fraction with Sephadex G-75, whereas the use of Sephadex G-50 or G-25 lead to a lower degree of purification. Thus the combination of the salting-out and the Sephadex G-75 methods resulted in a substrate concentration of approximately 1000 times that found in the untreated serum.
When after salting-out, the Sephadcx treatment was replaced by ultracentrifugation in a graded sugar-density-medium, the degree of purification was lower than with te Sephadex treat ent. However, three concentrat on-maxima at various degrees of sugar density were observed during the ultra-centrifugation, which suggested the presence of at least three different renin-substrates.
* l~it Unterstiitzung der Deutschen Forschungsgemeinschaft und des US Department of the Army, European Research Office.
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