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Unlocked Nucleic Acids with a Pyrene-Modified Uracil: Synthesis, Hybridization Studies, Fluorescent Properties and i-Motif Stability

✍ Scribed by Perlíková, Pavla; Karlsen, Kasper K.; Pedersen, Erik B.; Wengel, Jesper

Book ID: 125849001
Publisher: John Wiley and Sons
Year: 2013
Tongue: English
Weight: 384 KB
Volume: 15
Category: Article
ISSN: 1439-4227
DOI: 10.1002/cbic.201300567

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## Abstract Two pyrene‐modified UNA monomers were synthesized and incorporated into 21‐mer DNA oligonucleotides. Melting temperatures and thermodynamic properties of the modified duplexes were measured, and the fluorescence properties of single strands and duplexes containing one or more pyrene‐UNA