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Uniform labeling of a recombinant antibody Fv-fragment with 15N and 13C for heteronuclear NMR spectroscopy

โœ Scribed by Lutz Riechmann; John Cavanagh; Siobhan McManus

Book ID
115924378
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
396 KB
Volume
287
Category
Article
ISSN
0014-5793

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โœ Akira Ono; Shin-ichi Tate; Yoshiharu Ishido; Masatsune Kainosho ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 405 KB

13Cs]-2-Deoxy-D-ribose , synthesized from [13C6]-o-glucose (98% ~3C), was coupled with thymine to give [l',2',3',4',-thymidine (T) in an 18% overall yield. The thymidine was converted to the 3'-phosphoramidite derivative and was then incorporated into a dodecamer 5'-d(CGCGAATTCGCG)-3' by solid-phase