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Unfolding and refolding of ribonuclease T2 fromAspergillus oryzae

✍ Scribed by Yasushi Kawata; Kozo Hamaguchi

Book ID
105274065
Publisher
Springer
Year
1992
Tongue
English
Weight
226 KB
Volume
11
Category
Article
ISSN
1573-4943

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Using synthetic oligonucleotide probes, we have cloned a genomic DNA sequence encoding a ribonuclease (RNase) T2 gene (rntB) from Aspergillus oryzae on a 4.8 kb HindIII fragment. DNA sequence analysis of the RNase T2 revealed the following: (1) The gene is arranged as five exons and four introns; (2

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## Abstract The stability of ribonuclease T2 (RNase T2) from __Aspergillus oryzae__ against guanidine hydrochloride and heat was studied by using CD and fluorescence. RNase T2 unfolded and refolded reversibly concomitant with activity, but the unfolding and refolding rates were very slow (order of