Hydrogen exchange and the unfolding pathway of ribonuclease A
β Scribed by Thomas Kiefhaber; Robert L. Baldwin
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 596 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0301-4622
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## Abstract **Summary:** We use Fourier Transform infrared spectroscopy (FTβIR) spectroscopy to study the thermal unfolding and refolding behavior of ribonuclease (RNase A) adsorbed to spherical polyelectrolyte brushes (SPB). The SPB consist of a solid poly(styrene) core of ca. 100 nm diameter onto
The hydrogen-exchange reactions of peptide NH groups in lysozyme were studied by the change in the intensity of the amide I1 band in the ir spectrum. The slowest exchanging hydrogens, which are involved in intramolecular hydrogen bonding, are further divided into two groups a t lower temperatures; h
## Abstract __Differential scanning calorimetry, optical spectroscopy, and activity measurements were used to investigate the effect of mannosylglycerate, a negatively charged osmolyte widely distributed among thermophilic and hyperthermophilic archaea and bacteria, on the thermal unfolding of ribo