Comparative genomic hybridization (CGH) has been used widely for the molecular cytogenetic analysis of tumors. Until now, the spatial resolution of this technique for diagnosing deletions of chromosomal sequences has not been assessed in detail. In the present study, we performed CGH analyses on fiv
Unexpected detection of dystrophin gene deletions by array comparative genomic hybridization
β Scribed by Catherine E. Cottrell; Thomas W. Prior; Robert Pyatt; Caroline Astbury; Shalini Reshmi; Dennis Bartholomew; Joan Atkin; Kandamurugu Manickam; Devon Lamb Thrush; Matthew Pastore; Jerry Mendell; Chang-Yong Tsao; Roula al-Dahhak; Amy Newmeyer; Julie M. Gastier-Foster
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 264 KB
- Volume
- 152A
- Category
- Article
- ISSN
- 1552-4825
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At least 50 per cent of all first-trimester spontaneous abortions are cytogenetically abnormal, including trisomy, monosomy X, triploidy, tetraploidy and structural chromosome anomalies. Traditionally, the detection of aneuploidy in fetal tissues is performed by tissue sampling, cell culturing, meta
Comparative genomic hybridization (CGH) is a widely used technique for studying chromosomal imbalances. The sensitivity of the technique is, however, relatively low. Deletions down to a size of 10-12 Mbp have been detected by the use of fixed diagnostic thresholds. In this study, we applied standard