Deletions below 10 megabasepairs are detected in comparative genomic hybridization by standard reference intervals

✍ Scribed by Maria Kirchhoff; Tommy Gerdes; Jan Maahr; Hanne Rose; Martin Bentz; Hartmut Döhner; Claes Lundsteen

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 195 KB
• Volume: 25
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199908)25:4<410::aid-gcc17>3.0.co;2-j

✦ Synopsis

Comparative genomic hybridization (CGH) is a widely used technique for studying chromosomal imbalances. The sensitivity of the technique is, however, relatively low. Deletions down to a size of 10-12 Mbp have been detected by the use of fixed diagnostic thresholds. In this study, we applied standard reference intervals as detection criteria on a number of deletions in the range of 3 Mbp to 14-18 Mbp. All deletions were detected. Thus, detection by standard reference intervals confers a considerably higher sensitivity to CGH analysis compared to fixed diagnostic thresholds.