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Uneven distribution of protein kinase C-α and -β isozymes in human sarcomas and carcinomas

✍ Scribed by Antonio Cuadrado; Wolfgang Issing; Timothy P. Fleming; Christopher J. Molloy

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
842 KB
Volume
159
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Protein kinase C (PKC) represents a family of structurally related Ser/Tre kinases which are involved in mitogenic signalling and may contribute to human neoplasia. To address this issue, the messenger RNA and protein levels of PKC isoenzymes α and β were analyzed in several human sarcoma‐ and carcinoma‐derived cell lines. Carcinomas contained low or undetectable levels of either PKC‐α or PKC‐β. Sarcomas exhibited similar or increased PKC expression compared to human diploid fibroblasts. Moreover, sarcoma cell lines expressing one PKC isoform did not contain detectable levels of the other. When PKC was depleted from the tumor cells, we observed that the PKC overexpressing sarcomas had reduced their malignant properties as determined by their ability to grow in semisolid medium. In addition, epidermal growth factor‐stimulated and erbB2‐transformed fibroblasts exhibited enhanced cell growth in the absence of PKC. We propose a model for the effect of PKC as a negative regulator of proliferation in epithelial cells and a growth promoter in fibroblasts. © 1994 wiley‐Liss, Inc.

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